The term as it appears in Equation 10 (and various others) can be further optimised if a theoretical OTF is used. An experimentally obtained OTF requires its Fourier transform to be calculated in order for it to be convoluted with the smoothing function 4, this requires both memory and time. However, if a theoretical equation is obtained for , then the entire term may be evaluated theoretically. This will give an equation that can be used in the algorithm, in order to deconvolute Equation 10. Thus, a substantial amount of time and memory can be saved by using a theoretically computed OTF. Refer to Section 2.2 for details of what OTF was used.